cell signaling 2680  (Cell Signaling Technology Inc)

Journal: Acta Biochimica et Biophysica Sinica

Article Title: The effect of Scutellaria baicalensis stem-leaf flavonoids on spatial learning and memory in chronic cerebral ischemia-induced vascular dementia of rats

doi: 10.1093/abbs/gmw024

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: Treatment with 50 mg/kg SSTF did not show any noticeable effect on the expression of PP2A-sub B in both the cortex and hippocampus ( Table ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Type Dilution Specificity Phosphorylation sites Company pS199 Ploy- 1 : 1000 for WB P-tau Ser199 Biosource pS202 Ploy- 1 : 1000 for WB P-tau Ser202 Biosource pT205 Ploy- 1 : 1000 for WB P-tau Thr205 Biosource pS396 Ploy- 1 : 1000 for WB P-tau Ser396 Biosource pS404 Ploy- 1 : 1000 for WB P-tau Ser404 Biosource GSK3β Ploy- 1 : 1000 for WB Total GSK-3β Biovision p-GSK3β Ploy- 1 : 1000 for WB p-GSK3β Ser9 Biovision PP2A-sub B Ploy- 1 : 1000 for WB TotalPP2A subunit B Santa Cruz CDK5 1 : 1000 for WB Rabbit anti-CDK5 Cell Signaling Technology p35/25 1 : 1000 for WB Rabbit anti p35/25 Cell Signaling Technology Tau-5 Mono- 1 : 500 for WB Total-tau Abcam Open in a separate window Primary antibodies used in this study fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 6. caption a7 SSTF increased PP2A expression Representative immunoblots for the expression of PP2A subunit B (sub B) in the rat cortex (A) and hippocampus (B).

Techniques: Phospho-proteomics

Journal: Acta Biochimica et Biophysica Sinica

Article Title: The effect of Scutellaria baicalensis stem-leaf flavonoids on spatial learning and memory in chronic cerebral ischemia-induced vascular dementia of rats

doi: 10.1093/abbs/gmw024

Figure Lengend Snippet: SSTF reduced the expression levels of p35 and p25 Representative immunoblots showing the expression of Cdk5, p35 (Cdk5 activator), and p25 (p35 cleavage product) in the cortex (A) and the hippocampus (B). SSTF treatment (100 mg/kg) did not alter the expression of Cdk5 (C and D), but significantly reduced p35 and p25 in the VaD rat cortex (E and G) and hippocampus (F and H). The expression levels of Cdk5, p35, and p25 were normalized to GAPDH. The relative value of sham group was set as 100%. Data are shown as the mean ± SEM (n = 9). *P < 0.05 and **P < 0.01.

Article Snippet: Treatment with 50 mg/kg SSTF did not show any noticeable effect on the expression of PP2A-sub B in both the cortex and hippocampus ( Table ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Type Dilution Specificity Phosphorylation sites Company pS199 Ploy- 1 : 1000 for WB P-tau Ser199 Biosource pS202 Ploy- 1 : 1000 for WB P-tau Ser202 Biosource pT205 Ploy- 1 : 1000 for WB P-tau Thr205 Biosource pS396 Ploy- 1 : 1000 for WB P-tau Ser396 Biosource pS404 Ploy- 1 : 1000 for WB P-tau Ser404 Biosource GSK3β Ploy- 1 : 1000 for WB Total GSK-3β Biovision p-GSK3β Ploy- 1 : 1000 for WB p-GSK3β Ser9 Biovision PP2A-sub B Ploy- 1 : 1000 for WB TotalPP2A subunit B Santa Cruz CDK5 1 : 1000 for WB Rabbit anti-CDK5 Cell Signaling Technology p35/25 1 : 1000 for WB Rabbit anti p35/25 Cell Signaling Technology Tau-5 Mono- 1 : 500 for WB Total-tau Abcam Open in a separate window Primary antibodies used in this study fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 6. caption a7 SSTF increased PP2A expression Representative immunoblots for the expression of PP2A subunit B (sub B) in the rat cortex (A) and hippocampus (B).

Techniques: Expressing, Western Blot